Amidinoureas

ABSTRACT

A novel method of treating gastrointestinal, spasmolytic and ulcerogenic disorders by the administration of amidinoureas is disclosed.

This application is a continuation of copending application Ser. No. 942,247 filed Sept. 14, 1978 (now U.S. Pat. No. 4,204,000), which is a continuation-in-part application of Ser. No. 787,673, filed Apr. 14, 1977 (now abandoned), which is a divisional application of Ser. No. 558,187, filed Mar. 31, 1975 (now U.S. Pat. No. 4,060,635).

The present invention relates to a new method for treating gastrointestinal, spasmolytic and ulcerogenic disorders by the administration of amidinoureas.

BACKGROUND OF THE INVENTION

The pharmaceutical compositions which have been used as gastric antisecretory and spasmolytic agents have been such as atropine, homatropine, propantheline bromide, dicyclomine hydrochloride and other compounds which are structurally dissimilar to the biguanides of this invention. Due to the anticholinergic properties of these compounds, they are known to produce undesirable side effects such as mydriasis, xerostomia, cyclopegia and other unwanted effects.

There have been a number of 1-aryl biguanides described in the literature. They have been proposed for use as antidiabetics, anorexigenic or antimalarial agents. J. H. Burns and J. R. Vane, however, in the Brit. J. Pharmacol. (1948), 3:346-9 tested 1-(p-chlorophenyl)biguanide for its ability to reduce gastric secretion. Their findings determined that little or no reduction of gastric secretion was associated with this compound. Contrary to this belief:

We have unexpectedly found that certain amidinoureas possess valuable pharmacologic properties and these compounds then unexpectedly possess useful gastric antisecretory and spasmolytic properties.

We have also found that these amidinoureas are substantially free of the anticholinergic side effects which accompany gastric antisecretory and spasmolytic agents.

We have further found that these amidinoureas have a low order of toxicity.

We have still further found a simple and effective method for treating gastrointestinal disorders and diseases, such as duodenal and peptic ulcers.

DESCRIPTION AND PREFERRED EMBODIMENTS

The present invention describes a method for treating gastrointestinal hyperacidity, gastrointestinal spasms or gastrointestinal ulcerations in human or mammal in need thereof by the oral or parenteral administration of an effective amount of an amidinourea of formula I or its tautomeric form II when R is hydrogen: ##STR1## where: R₂, R₃, R₄, R₅ and R₆ may be the same or different and are:

hydrogen,

halo,

loweralkyl,

haloloweralkyl,

nitro,

loweralkoxy,

hydroxy,

arloweralkoxy,

acyloxy,

cyano,

haloloweralkoxy or

loweralkylsulfonyl;

R and R' are hydrogen or loweralkyl;

R" and R'" are hydrogen,

loweralkyl,

loweralkenyl,

cycloalkenyl,

cycloalkylloweralkyl,

cycloalkyl,

aralkyl,

loweralkynyl,

haloalkyl,

hydroxyalkyl,

alkoxyalkyl,

cyanoalkyl,

aminoalkyl,

mono- and di-loweralkylaminoalkyl,

carbamoylalkyl,

mono- and di-carbamoylalkyl,

carboxyalkyl,

alkoxycarbonylalkyl,

aralkoxycarbonylalkyl,

formyl,

acyl,

acylalkyl,

alkylsulfonyl or

aralkylsulfonyl;

R" and R'" together may form a 5-7 atom ring which may include 0-2 hetero atoms of N, O or S;

R_(n) is hydrogen or it may be loweralkyl provided at least one of R, R', R" and R'" is other than hydrogen; and

the non-toxic acid addition salts thereof.

Compounds of this invention which are preferred include those where:

R₂, R₃, R₄, R₅ and R₆ are hydrogen,

halo,

loweralkyl,

haloloweralkyl,

nitro,

hydroxy or

loweralkoxy; and

R' and R_(n) are hydrogen or loweralkyl and

R" and R'" are hydrogen or alkyl; provided R, R', R" and R'" are not all hydrogen at the same time.

The compounds of formula I or II of the present invention exist as tautomeric forms when a proton is present on the second nitrogen atom of the amidinourea chain. As with tautomers certain compounds would naturally exist in one form or the other depending on their character as discussed in Journal of Organic Chemistry, 33, 1968 p. 552.

The more preferred compounds of this invention include those where:

R₂ is hydrogen or loweralkyl;

R₃ and R₅ are hydrogen, hydroxy or loweralkoxy;

R₄ is hydrogen,

loweralkyl,

hydroxy,

loweralkoxy or

halo;

R₆ is hydrogen,

loweralkyl,

nitro,

alkoxy or

halo;

R and R_(n) are hydrogen or loweralkyl; and

R' and R" are hydrogen or alkyl; provided R, R', R" and R'" are not all hydrogen at the same time.

The most preferred compounds of this invention are those where:

R₂ is hydrogen,

methyl or

ethyl;

R₃ is hydrogen,

hydroxy or

methoxy;

R₄ is hydrogen,

methyl,

ethyl,

hydroxy,

methoxy,

chloro or

bromo;

R₅ is hydrogen,

hydroxy or

methoxy;

R₆ is hydrogen,

methyl,

ethyl,

nitro,

methoxy,

ethoxy,

chloro,

bromo or

fluoro;

R and R_(n) are hydrogen,

methyl or

ethyl; and

R' and R" are hydrogen,

methyl,

ethyl,

propyl,

i-propyl,

butyl,

i-butyl,

sec-butyl,

t-butyl,

pentyl,

hexyl or;

heptyl; provided R, R', R" and R'" are not all hydrogen at the same time.

A special embodiment of this invention comprises compounds which have:

R₂ -loweralkyl substitution;

R₂, R₆ -diloweralkyl substitution;

R₂, R₆ -loweralkyl, alkoxy substitution;

R₂, R₆ -loweralkyl, halo substitution;

R₂, R₆ -alkyl, nitro substitution;

R₂, R₄, R₆ -triloweralkyl substitution, or

R₂, R₄, R₆ -loweralkyl, dihalo substitution.

A further special embodiment of this invention comprises compounds which have:

R₃, R₄ -hydroxy or alkoxy substitution;

R₃, R₄, R₅ -hydroxy or alkoxy substitution;

R₂, R₅ -dihalo substitution or

R₂, R₆ -dihalo substitution.

A further special embodiment of this invention comprises compounds which have:

R, R', R" and R'" as hydrogen or loweralkyl substitution provided all are not hydrogen at the same time; or

R and R' are hydrogen or loweralkyl and R" and R'" are an alkyl group from 3 to 7 carbon atoms.

The compounds of formula I form the subject matter of U.S. Pat. No. 4,060,635, Nov. 29, 1977 and this is incorporated herein by reference. The compounds of formula I listed in U.S. Pat. No. 4,060,637 include:

1-(N,N-pentamethyleneamidino)-3-(2,6-dimethyl phenyl)urea

1-[(N,N-(N-methyl-3'-azapentamethylene)amidino]-3-(2,6-dimethylphenyl)urea

1-[N,N-(N-methyl-3'-azaheptamethylene)amidino]-3-(2,6-dimethylphenyl)urea

1-[N,N-(3'-oxopentamethylene)amidino]-3-(2,6-dimethylphenyl)urea

1-[N,N-(2'-thiatetramethylene)amidino]-3-(2,6-dimethylphenyl)urea.

The compounds corresponding to formula I are useful in treating gastrointestinal disorders such as hyperacidity, spasms and peptic and gastric ulcers. For these purposes they can be eliminated orally, parenterally or rectally. Administration by the oral route is preferred. Orally, these compounds may be administered in tablets, hard or soft capsules, aqueous or oily suspensions, dispersible powders or granules, emulsions, syrups or elixers. The optimum dosage, of course, will depend on the particular compound being used and the type and severity of the condition being treated. In any specific case, the appropriate dosage selected will further depend on factors of the patient which may influence response to the drug; for example, general health, age, weight, etc. of the subject being treated.

Although the optimum quantities for administration of the compounds I in accordance with the present invention will depend on the compound employed and the particular type of disease condition treated, oral dose levels of preferred compounds when administered to a mammal in dosages of 0.05 to 50 milligrams per kilogram of body weight per day are particularly useful. The preferred range is 0.1 to 20 mg/kg. Comparative dosages may be used in parenteral or rectal administration.

Compositions intended for oral use may be prepared according to methods known to the art for the manufacture of pharmaceutical compositions. Such compositions may contain one or more agents selected from the group consisting of sweetening agents, flavoring agents, coloring agents, preserving agents, etc. in order to provide a pharmaceutically elegant and palatable preparation.

Further, the active amidinourea may be administered alone or in admixture with other agents having the same or different pharmacological properties.

The composition may contain such selected excipients such as inert diluents such as calcium carbonate, lactose, etc.; granulating and disintegrating agents such as maize starch, alginic acid, etc.; lubricating agents such as magnesium stearate, etc.; binding agents such as starch gelatin; etc.; suspending agents such as methylcellulose, vegetable oil, etc.; dispersing agents such as lecithin, etc.; thickening agents such as beeswax, hard paraffin, etc.; emulsifying agents such as naturally-occurring gums, etc.; non-irritating excipients such as cocoa butter, polyethylene glycols, etc.; and the like. Further, in formulating these compounds for every 100 parts by weight of the composition, there may be present between 5 and 95 parts by weight of the active ingredient. The dosage unit form will generally contain between 0.1 mg. and about 500 mg. of the active ingredients of this invention. The preferred unit dose is between 1 mg. and about 50 mg. The compositions may be taken 1-8 times daily depending on the dosage unit required.

The dosage regimen in carrying out the methods of this invention is that which insures maximum therapeutic response until improvement is obtained and thereafter the minimum effective level which gives relief. Thus, in general, the dosages are those that are therapeutically effective in the treatment of ulcerogenic disease conditions or symptoms, such as duodenal and peptic ulcer. In general, the daily dose can be between about 0.1 mg/kg and 70 mg/kg (preferably in the range of 1-25 mg/kg/day), bearing in mind, of course, that in selecting the appropriate dosage in any specific case, consideration must be given to the patient's weight, general health, age and other factors which may influence response to the drug.

Various tests in animals have been carried out to show the ability of the compounds of this invention to exhibit reactions that can be correlated with anti-ulcerogenic activity in humans. These tests involve such as the effect of the amidinoureas of the formula I on gastric secretion, gastrointestinal spasm and their mucogenic effect. It has been found that the compounds of this invention when tested in the above variety of situations, show a marked activity.

One such test is the gastric secretion test. This test is carried out as follows: Shay rats are fasted for 4-8 hours, and water is given ad lib. The rats are selected at random and separated into groups of 10. The animals are treated intraduodenally (I.D.) with the test compound or the vehicle immediately subsequent to the ligation of the stomach at the pyloric sphincter. The animals are sacrificed with chloroform at 4 hours post-drug administration, the stomach is removed and its contents are assayed for volume, pH and total acids.

A second gastric secretion test is carried out on dogs. This is outlined in the Handbook of Physiology, Section 6: Alimentary Canal, Volume II: Secretion, American Physiology Society, Washington, D.C., 1967.

It has been found that the compounds of this invention when subjected to the above gastric secretion tests display a marked ability to decrease gastric volume and gastric acidity. These tests are known to correlate well with gastric activity in humans and is a standard test used to determine antisecretory properties.

To determine the anti-ulcer effectiveness the following test is employed: Male Wistar rats (130-150 grams) are fasted for 24 hours, then given reserpine at 5 mg/kg i.p. Twenty-four hours later, the stomachs are removed and examined for ulceration. Ulcers are graded on a 0-4 scale and the number of ulcers is recorded. Pretreatment with the 1,5-disubstituted biguanide compounds of this invention produces a decrease in ulcer grade and the number of ulcers compared to the control reserpine-treated rats.

Determination of antispasmodic properties can be carried out by the procedure as outlined by D. A. Brodie and S. K. Kundrats in their article entitled "Effect of Drugs on Gastric Emptying in Rats", Fed. Proc. 24:714 (1965).

The biguanides of this invention have also been found to be mucogenic agents, that is, they increase the biosynthesis of mucopolysaccharides of the gastric mucous membrane which is a mechanism for inhibiting gastrointestinal ulcer. This property is determined by the test outlined in the J. Pharm. Pharmac., 1970, 22, 143-4.

Mydriasis is detected by the procedure R. A. Turner, Screening Methods in Pharmacology, Academic Press, New York, and London, pp. 174-5, 1965. Acute toxicity is calculated according to the standard Litchfield-Wilcoxon procedure.

In view of the results of these tests the pharmacological data clearly indicates that the amidinoureas of the formula I substituted biguanides of this invention can be considered to be effective anti-ulcerogenic agents having active gastric antisecretory and antispasmodic properties which are substantially free of anticholinergic side effects and having a low toxicity. 

We claim:
 1. A method for treating gastrointestinal hyperacidity in humans and mammals in need thereof which comprises the oral or parenteral administration of an effective amount of an amidinourea of the general formula I or its tautomeric form II when R is hydrogen: ##STR2## where: R₂, R₃, R₄, R₅ and R₆ may be the same or different and arehydrogen, halo, loweralkyl, trifluoromethyl, nitro, loweralkoxy, or methylsulfonyl, provided that they are not all hydrogen at the same time; R and R' are hydrogen or loweralkyl; R" and R'" are hydrogen,loweralkyl, loweralkenyl, cycloalkenyl up to 9 carbon atoms, cycloalkylloweralkyl, loweralkyl, cycloalkyl, aralkyl, loweralkynyl, haloalkyl, hydroxyalkyl, alkoxyalkyl, cyanoalkyl, aminoalkyl, mono- and di-loweralkylaminoalkyl, carbamoylalkyl, mono- and di-carbomoylalkyl, carboxyalkyl, alkoxycarbonylalkyl, aralkoxycarbonylalkyl, formyl, acyl, acylalkyl, alkylsulfonyl or aralkylsulfonyl; R" and R'" together may form a 5-7 atom ring which may include 0-2 hetero atoms of N, O or S; R_(n) is hydrogen or loweralkyl provided at least one of R, R', R" and R'" is other than hydrogen; and the non-toxic acid addition salts thereof.
 2. The method of treating gastrointestinal spasm in humans and mammals in need thereof which comprises the oral or parenteral administration of an effective amount of an amidinourea of the general formula I or its tautomeric form II where R is hydrogen: ##STR3## where: R₂, R₃, R₄, R₅ and R₆ may be the same or different and arehydrogen, halo, loweralkyl, trifluoromethyl, nitro, loweralkoxy, or methylsulfonyl, provided they are not all hydrogen at the same time; R and R' are hydrogen or loweralkyl; R" and R'" are hydrogen,loweralkyl, loweralkenyl cycloalkenyl up to 9 carbon atoms, cycloalkylloweralkyl, loweralkyl, cycloalkyl, aralkyl, loweralkynyl, haloalkyl, hydroxyalkyl, alkoxyalkyl, cyanoalkyl, aminoalkyl, mono- and di-loweralkylaminoalkyl, carbamoylalkyl, mono- and di-carbomoylalkyl, carboxyalkyl, alkoxycarbonylalkyl, aralkoxycarbonylalkyl, formyl, acyl, acylalkyl, alkylsulfonyl or aralkylsulfonyl; R" and R'" together may form a 5-7 atom ring which may include 0-2 hetero atoms of N, O or S; R_(n) is hydrogen or loweralkyl provided at least one of R, R', R" and R'" is other than hydrogen; and the non-toxic acid addition salts thereof.
 3. A method for treating gastrointestinal ulceration in humans and mammals in need thereof which comprises the oral or parenteral administration of an effective amount of an amidinourea of the general formula I or its tautomeric form II when R is hydrogen: ##STR4## where: R₂, R₃, R₄, R₅ and R₆ may be the same or different and arehydrogen, halo, loweralkyl, trifluoromethyl, nitro, loweralkoxy, or methylsulfonyl, provided they are not all hydrogen at the same time; R and R' are hydrogen or loweralkyl; R" and R'" are hydrogen,loweralkyl, loweralkenyl cycloalkenyl up to 9 carbon atoms, cycloalkylloweralkyl, loweralkyl, cycloalkyl, aralkyl, loweralkynyl, haloalkyl, hydroxyalkyl, alkoxyalkyl, cyanoalkyl, aminoalkyl, mono- and di-loweralkylaminoalkyl, carbamoylalkyl, mono- and di-carbomoylalkyl, carboxyalkyl, alkoxycarbonylalkyl, aralkoxycarbonylalkyl, formyl, acyl, acylalkyl, alkylsulfonyl or aralkylsulfonyl; R" and R'" together may form a 5-7 atom ring which may include 0-2 hetero atoms of N, O or S; R_(n) is hydrogen or loweralkyl provided at least one of R, R', R" and R'" is other than hydrogen; and the non-toxic acid addition salts thereof.
 4. The method of claim 1 wherein R₂, R₃, R₄, R₅ and R₆ may be the same or different and arehydrogen, fluoro, chloro, bromo, methyl, ethyl, propyl, isopropyl, butyl, trifluoromethyl, nitro, methoxy, ethoxy, or methylsulfonyl,provided they are not all hydrogen at the same time.
 5. The method of claim 2 wherein R₂, R₃, R₄, R₅ and R₆ may be the same or different and arehydrogen, fluoro, chloro, bromo, methyl, ethyl, propyl, isopropyl, butyl, trifluoromethyl, nitro, methoxy, ethoxy, or methylsulfonyl,provided they are not all hydrogen at the same time.
 6. The method of claim 3 wherein R₂, R₃, R₄, R₅ and R₆ may be the same or different and arehydrogen, fluoro, chloro, bromo, methyl, ethyl, propyl, isopropyl, butyl, trifluoromethyl, nitro, methoxy, ethoxy, or methylsulfonyl,provided they are not all hydrogen at the same time.
 7. The method of claim 1 wherein:R and R' are hydrogen or loweralkyl, provided R and R' are not all hydrogen at the same time; R" and R'" are hydrogen,loweralkyl, loweralkenyl, cycloalkenyl up to 9 carbon atoms, cycloalkylloweralkyl, loweralkyl, cycloalkyl, aralkyl, loweralkynyl, haloalkyl, hydroxyalkyl, alkoxyalkyl, cyanoalkyl, aminoalkyl, mono- and di-loweralkylaminoalkyl, carbamoylalkyl, mono- and di-carbamoylalkyl, carboxyalkyl, alkoxycarbonylalkyl, aralkoxycarbonylalkyl, formyl, acyl, acylalkyl, alkylsulfonyl or aralkylsulfonyl, provided R" and R'" are not all hydrogen at the same time.
 8. The method of claim 2 wherein:R and R' are hydrogen or loweralkyl, provided R and R' are not all hydrogen at the same time; R" and R'" are hydrogen,loweralkyl, loweralkenyl, cycloalkenyl up to 9 carbon atoms, cycloalkylloweralkyl, loweralkyl, cycloalkyl, aralkyl, loweralkynyl, haloalkyl, hydroxyalkyl, alkoxyalkyl, cyanoalkyl, aminoalkyl, mono- and di-loweralkylaminoalkyl, carbamoylalkyl, mono-and di-carbamoylalkyl, carboxyalkyl, alkoxycarbonylalkyl, aralkoxycarbonylalkyl, formyl, acyl, acylalkyl, alkylsulfonyl or aralkylsulfonyl, provided R" and R'" are not all hydrogen at the same time.
 9. The method of claim 3 wherein:R and R' are hydrogen or loweralkyl, provided R and R' are not all hydrogen at the same time; R" and R'" are hydrogen,loweralkyl, loweralkenyl, cycloalkenyl up to 9 carbon atoms, cycloalkylloweralkyl, loweralkyl, cycloalkyl, aralkyl, loweralkynyl, haloalkyl, hydroxyalkyl, alkoxyalkyl, cyanoalkyl, aminoalkyl, mono- and di-loweralkylaminoalkyl, carbamoylalkyl, mono- and di-carbamoylalkyl, carboxyalkyl, alkoxycarbonylalkyl, aralkoxycarbonylalkyl, formyl, acyl, acylalkyl, alkylsulfonyl or aralkylsulfonyl, provided R" and R'" are not all hydrogen at the same time. 